mouse anti-beta-tubulin-iii (3f3-g2) sc-53140  (Santa Cruz Biotechnology)

Journal: eLife

Article Title: Regulative synthesis of capsular polysaccharides in the pathogenesis of Streptococcus suis

doi: 10.7554/eLife.101760

Figure Lengend Snippet: Mice were inoculated with acetic acid through the nostril, and 1 hr later, infected intranasally (i.n.) with 05ZYH33. ( A ) Sagittal views of the olfactory system. Sections of the distal nasal cavity and olfactory epithelium from uninfected (a, c, and e) and 05ZYH33-infected (b, d, and f) mice. Red or orange, 05ZYH33; green, the neuronal marker β-tubulin III; blue, DNA. NC, nasal cavity; OE, olfactory epithelium; LP, lamina propria; CP, cribriform plate; OB, olfactory bulb. ( B ) A schematic drawing of the sagittal plane of the rodent nose elucidates the compartments of the olfactory bulb (OB), olfactory epithelium (OE), nasal cavity (NC), and brain. This panel is redrawn from ‘Mouse Olfactory System’ ( inspiredpencil.com ). The dotted line indicates the anteroposterior localization of the coronal sections in C. ( C ) One hour or nine days after 05ZYH33 infection, coronal brain sections were prepared and stained with hematoxylin and eosin (H&E). The sections display the regions of the ventral striatum and basal forebrain located behind the anterior olfactory nucleus. Arrows indicate infiltrated inflammatory cells in the lower areas of the ventral striatum or basal forebrain. ( D, E ) Mice were inoculated with acetic acid or phosphate-buffered saline (PBS), and 1 hr later infected i.n. with 05ZYH33 or ∆2BSS2. ( D ) Colony-forming units (CFUs) of 05ZYH33 or ( E ) ∆2BSS2 in nasal-associated lymphoid tissue (NALT), the cerebrospinal fluid (CSF), and blood were determined 1 hr after infection.

Article Snippet: Immunostaining was conducted using a primary rabbit anti- S. suis antibody (1:100) and a mouse anti-tubulin beta III antibody (Merck Millipore, Darmstadt, Germany catalog no. mab5564, 1:300) to detect neuronal structures, and then appropriate fluorophore-conjugated secondary antibodies: donkey anti-rabbit Cy3 (Jackson ImmunoResearch Laboratories, Ely, UK, catalog no. 711-166-152, 1:1000), donkey anti-mouse FITC (Jackson ImmunoResearch Laboratories, Ely, UK, catalog no. 715-096-150, 1:500).

Techniques: Infection, Marker, Staining, Saline

Journal: Cell reports

Article Title: Cell-type-specific roles of FOXP1 in the excitatory neuronal lineage during early neocortical murine development

doi: 10.1016/j.celrep.2025.115384

Figure Lengend Snippet:

Article Snippet: mouse anti-neuronal class III β-tubulin/Tuj1 , Covance , Cat# MMS-435P.

Techniques: Recombinant, Electron Microscopy, RNAscope, Isolation, Software, Microscopy

Journal: Human Cell

Article Title: Novel CRISPR-Cas9 iPSC knockouts for PCCA and PCCB genes: advancing propionic acidemia research

doi: 10.1007/s13577-025-01193-z

Figure Lengend Snippet: Antibodies used in the study

Article Snippet: Mouse IgG anti-β-III-Tubulin Tuj1 , 1:500 , Covance Cat# MMS-435P, AB_231377.

Techniques: Immunocytochemistry, Cytometry, Western Blot

Journal: Human Cell

Article Title: Novel CRISPR-Cas9 iPSC knockouts for PCCA and PCCB genes: advancing propionic acidemia research

doi: 10.1007/s13577-025-01193-z

Figure Lengend Snippet: Characterization of the PCCA and PCCB KO iPSC lines. A Representative phase contrast images of iPSC colonies in both KO iPSC lines (scale bar 40 µm). B Representative G-banding karyotype images of normal 46, XX for both cell lines. C Immunofluorescence analysis for OCT4/NANOG/SOX2/SSEA-3/SSEA-4/TRA-1–60 and TRA-1–81 pluripotency markers (scale bar 40 µm). D Flow cytometry analysis for SSEA-4/TRA-1–60 and TRA-1–81 surface pluripotency markers. E In vitro differentiation analysis by immunofluorescence for AFP, SMA and TUJ1 proteins (scale bar 40 µm)

Article Snippet: Mouse IgG anti-β-III-Tubulin Tuj1 , 1:500 , Covance Cat# MMS-435P, AB_231377.

Techniques: Immunofluorescence, Flow Cytometry, In Vitro